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Fig. 7. The P2Y₂R accesses G₁₂ in a complex with ${alpha}$ v integrins. (A) RGD-dependent association of the P2Y₂R with ${alpha}$ v integrins and G ${alpha}$ ₁₂. Cells expressing the HA-tagged WT or RGE mutant P2Y₂R and endogenous G_q/11, G₁₂ and ${alpha}$ v integrins were treated with or without 1 mM UTP for 5 minutes and lysates were subjected to IP with mouse anti-HA antibody conjugated to agarose beads and IB with anti-G ${alpha}$ ₁₂, anti-G ${alpha}$ _q/11, anti- ${alpha}$ v integrin, or anti-HA antibody. Cells transfected with the pLXSN vector served as a negative control. (B) G ${alpha}$ ₁₂ associates with ${alpha}$ v integrins. Human 1321N1 cells expressing cDNA for the WT P2Y₂R and G ${alpha}$ ₁₂ were treated with or without 100 µM UTP for 5 minutes and lysates were subjected to IP with IgG, anti- ${alpha}$ 3 integrin, or anti- ${alpha}$ v integrin antibody followed by IB with anti-G ${alpha}$ ₁₂, anti- ${alpha}$ 3 integrin or anti- ${alpha}$ v integrin antibody. Blots representative of three experiments are shown.

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