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Figure 2


Fig. 2. GTPase binding of IRSp53 and IRTKS. GST-fused constitutively active (L61) or dominant negative (N17) Cdc42 and Rac bound to glutathione beads were incubated with extract from COS7 cells expressing Myc-tagged constructs (as indicated) or with untransfected C2C12 extract (endogenous). Bead-bound material was analysed by immunoblotting alongside an equivalent fraction of the original extracts. Blots were probed with anti-Myc (9E10). FL, full-length protein; aa, amino acid residues present in truncated constructs. IRTKS exhibits IMD-mediated binding to both Rac mutants.





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