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Figure 4


Fig. 4. Cell area changes in cells expressing IRSp53 or IRTKS Myc-tagged IRTKS, IRSp53-S, IRSp53-L, IRSp53+Ct or GFP (control) were expressed in COS7 cells as shown. IRSp53-S and IRSp53-L constructs decreased the area of the cell body in contrast to IRTKS and IRSp53+Ct. IRSp53-S and IRSp53-L have significantly reduced cell body areas compared with COS7 cells overexpressing only GFP. The graph shows the mean of three independent experiments in which 30 cells were measured for each construct per experiment ± s.e.m. *P<0.05 compared with GFP control cells. The cell body area is drawn in red on each image. Bar, 10 µm.





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