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Figure 1


Fig. 1. Hypertonic incubation causes compaction of chromatin in living cells: MCF7 cells were incubated for 20 minutes with growth medium enriched with varying concentrations sucrose as indicated. The cells were fixed and stained with ToPro3 to observe the chromatin distribution by confocal laser scanning microscopy. The confocal sections demonstrate the progress in chromatin compaction with increasing load of sucrose. Neither chromosome bands nor individual chromosomes became discernible. Bar, 10 µm.





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