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Figure 2


Fig. 2. Activity of the Ire1 mutants as measured by a UPRE-lacZ reporter. KMY1015 (ire1 null mutant) cells carrying both pCZY1 (UPRE-lacZ reporter plasmid) and pRS315-Ire1-HA or mutant alleles were cultured with (+) or without (–) 2 µg/ml tunicamycin (Tun) for 4 hours and subjected to beta-galactosidase assay. For the empty vector control ({Delta}ire1), pRS315 was used instead of pRS315-Ire1-HA. In all three panels, each value is the mean ± s.d. for three independent clones and was normalized to that of the Tun+ wild-type Ire1-HA control, which was set at 100.





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