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Files in this Data Supplement:
Movie 1. Direct adenylyl cyclase stimulation using forskolin does not initiate gap formation in control PMVEC monolayers. 1 µmol/l (µM) forskolin was added at time 0 (minutes) and images taken every 2 minutes for 1 hour. Image sequences are displayed at 5 frames/second. Movie 1 corresponds to Fig. 7, first panel.
Movie 2. Direct adenylyl cyclase stimulation using forskolin initiates gap formation in PMVECs expressing the catalytically inactive PDE4D4(1-166)/GFP peptide. 1 µmol/l (µM) forskolin was added at time 0 (minutes) and images taken every 2 minutes for 1 hour. Image sequences are displayed at 5 frames/second. Movie 2 corresponds to Fig. 7, second panel.
Movie 3. Direct adenylyl cyclase stimulation using forskolin does not initiate gap formation in vector control PMVEC monolayers. 1 µmol/l (µM) forskolin was added at time 0 (minutes) and images taken every 2 minutes for 1 hour. Image sequences are displayed at 5 frames/second. Movie 3 corresponds to Fig. 7, fourth panel.
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