spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 6


Fig. 6. PKA-mediated phosphorylation of tau at Ser214 reorganizes microtubules. (A) Forskolin (1 µM, 30 minutes) increased co-immunoprecipitation of phosphorylated tau-Ser214 with β-tubulin in the cytosol of PDE4D41-166-expressing PMVECs, which was not seen in vector controls. Such enhanced co-immunoprecipitation of phosphorylated tau with β-tubulin was not observed using the phosphorylated tau-Ser262 antibody under the same conditions. (B) The forskolin-stimulated increase in tau-Ser214 phosphorylation was prominent in the depolymerized microtubule-enriched fractions, and was not observed in polymerized fractions. (C) H89 pretreatment (10 µM, 10 minutes before forskolin stimulation) blocked the forskolin-induced phosphorylation of tau-Ser214 binding to β-tubulin in cytosol. (D) Phosphorylated tau-Ser214 (red) and microtubules (green) are shown in control and PDE4D41-166-expressing PMVECs. Whereas PDE4D41-166 did not influence resting tau-Ser214 or microtubule distribution (top panel), the application of forskolin (bottom panel) abruptly reorganized microtubules into bundles. (White boxes denote area enlarged in right panel of each image set.)





Right arrow Return to article