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Figure 4


Fig. 4. Development of cGMP-mutant cell strains. Mutant cells were starved as a monolayer on non-nutrient agar plates. Pictures of developing cells were taken at 5 hours after the start of starvation. gc-null cells lack cGMP synthesis, whereas gpbC-null cells lack the cGMP-target protein GbpC that mediates cell polarity. sGC{Delta}N, expressed in gc-null cells, is catalytically active but cytosolic. sGC{Delta}cat is catalytically inactive but localizes to the anterior of the cell, an event that previously was shown to stabilize the leading edge in a spatial gradient.





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