spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 2


Fig. 2. Analysis of tyrosine phosphorylation of other junction proteins in ICAM1 stimulated ECs. (A-E) Confluent GPNT cells were serum starved and ICAM1 crosslinked (XL). At the indicated times cells were washed, lysed and subjected to immunoprecipitation of (A) ZO-1 and (B-E) catenins as indicated. Immunoprecipitates were then analyzed by immunoblotting using antibodies against phosphorylated tyrosine, ZO-1, VEC or catenin. Black and white arrowheads in A-E indicate the position of migration of VEC and relevant catenins, respectively, as determined by stripping and re-probing of the immunoblots. Immunoprecipitates of p120 did not contain detectable VEC, whether phosphorylated (black arrowhead) or not (data not shown). (F) The chicken occludin-expressing GPNT cell line (see supplementary material Fig. S1) was grown to confluence, serum starved and ICAM1 crosslinked (XL). At the indicated times chicken occludin was immunoprecipitated and analyzed by immunoblotting for phosphorylated tyrosine or occludin. In all blots the position of size markers (in kDa) is indicated on the left.





Right arrow Return to article