spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 3


Fig. 3. Plo1p binds directly to and phosphorylates Mbx1p in vitro. (A) In vitro kinase assay with purified 6His-Plo1p and recombinant components of PBF. Separate kinase reactions with 6His-Plo1p and either 6His-Fkh2p, GST-Sep1p or GST-Mbx1p were separated by SDS PAGE, stained with Coomassie Blue and autoradiographed. Arrow indicates phosphorylated GST-Mbx1p, near to autophosphorylated 6His-Plo1p. Table indicates combinations of tagged proteins used. (B) In vitro kinase assay with purified 6His-Plo1p and GST-Mbx1p bound to glutathione-Sephadex beads after washes with 1% NP-40 to remove 6His-Plo1p, separated by SDS PAGE, stained with Coomassie Blue and autoradiographed. Arrow indicates phosphorylated GST-Mbx1p. Table indicates combinations of tagged proteins used.





Right arrow Return to article