JCS014522 Supplementary Material

Files in this Data Supplement:

• Movie 1 -

  Movie 1. First example of a cell displaying spontaneous Ca²⁺_c oscillations in which two mitochondria depolarize. Colonic myocytes were co-loaded with Fluo-4 (left-hand panel, green-yellow psuedocolor to indicate intensity) and TMRE (right-hand panel, red-yellow psuedocolor to indicate intensity) and voltage-clamped at −70 mV in the whole-cell configuration. Fluorescent images were collected at 1 Hz over 10 minutes. Scale bar, 10 μm.

• Movie 2 -

  Movie 2. Spontaneous Ca²⁺_c oscillations and changes in ΔΨ_m; second example: cell in which many mitochondria depolarize. Colonic myocytes were co-loaded with Fluo-4 (left-hand panel, green-yellow psuedocolor to indicate intensity) and TMRE (right-hand panel, red-yellow psuedocolor to indicate intensity) and voltage-clamped at −70 mV in the whole-cell configuration. Fluorescent images were collected at 1 Hz over 10 minutes. Scale bar, 10 μm.