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Figure 6


Fig. 6. Neither Ins(1,4,5)P3 (IP3)- nor ICa-induced [Ca2+]c increases altered the {Delta}{Psi}m of the entire-cell mitochondrial complement when measured with TMRE in `quench' mode. {Delta}{Psi}m depolarization was not seen during increases of [Ca2+]c induced by either Ins(1,4,5)P3 (released by localized photolysis of caged Ins(1,4,5)P3, at {downarrow}, A), following a single plasmalemmal depolarization (+10 mV for 500 ms, B) or following a train of plasmalemmal depolarizations (+10 mV for 250 ms, 2 Hz, 5 seconds, C) in myocytes co-loaded with Fluo-4 (10 µM) and TMRE (150 nM, see text). By contrast, the mitochondrial inhibitors CCCP plus oligomycin (1 and 6 µM, respectively, D) significantly increased TMRE fluorescence when compared with untreated controls (E); **P<0.01; n.s. (not significant) P>0.05.





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