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Figure 4


Fig. 4. Immunofluorescence staining of wild-type T. brucei, Leishmania and Leishmania-transfectant cells. (A) T. brucei procyclic cells and (B) Leishmania promastigotes were stained with antibodies against TbEG ({alpha}-rTbEG) as primary, and FITC-conjugated anti-rabbit IgG as secondary, antibodies. To visualize mitochondria, cells were labeled with Mitotracker Red (Mito Red). Pre-immune serum (NRS) was used in control staining experiments. (C) Axenic amastigotes overexpressing endoG (LdEG) were labeled with antibodies against the HA epitope ({alpha}-HA). Mitochondria were labeled with Mitotracker Red. LdKS transfectants were used as a control. Bars, 5 µm (A); 2.5 µm (B,C).





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