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Fig. 7. H₂O₂-triggered release of endoG from mitochondria. (A) L. donovani axenic amastigote transfectants (LdKS, LdEG) treated with or without H₂O₂ under the conditions used to trigger cell death were incubated in a buffer containing digitonin, and fractions representing cytosol (C) and mitochondria (M) were analyzed by SDS-PAGE and western blotting using antibodies against HA ( ${alpha}$ -HA), T. brucei endoG ( ${alpha}$ -rTbEG) and Leishmania cytochrome c ( ${alpha}$ -LdCyt C). The blots were re-probed with antibodies against T. brucei Hsp70 ( ${alpha}$ -TbHsp70) and Leishmania adenine phosphoribosyltransferase ( ${alpha}$ -LdAPRT). (B) Western blot showing the cross-reactivity of the antibody against TbHsp70 with Leishmania lysates.

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