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Figure 3


Fig. 3. Subcellular localization of wild-type Wingless and [C93A]Wingless in ectodermal cells of third-instar larvae. (A-C) High-magnification view of a region of ectodermal cells co-expressing wild-type Wingless from two transgenes (one tagged with GFP and the other with HA) with the wg-Gal4 driver. Inset in A shows the whole cell. GFP is shown in green and HA in red. Extensive overlap is seen (yellow in A), including in large structures (arrowheads). (D-F) Co-expression of GFP-tagged wild-type Wingless (green) with HA-tagged [C93A]Wingless (red). Again, the whole cell is shown in the inset in D. Only partial overlap is seen between the mutant and wild-type proteins (D). Note, for example, the lack of colocalisation in large structures (arrowheads). Scale bars: 1 µm.





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