JCS023119 Supplementary Material

Files in this Data Supplement:

• Supplemental Figure S1 (Adobe PDF) -

  Fig. S1. Primers used for gene construction.

• Supplemental Figure S2 (Adobe PDF) -

  Fig. S2. Subcellular localization of Zfp64. (A) Constructs encoding GFP-tagged Zfp64, CSL and NICD were transfected into U2OS cells. After 48 hours, cells were fixed in methanol and counterstained with DAPI. GFP-Zfp64, GFP-CSL and GFP-NICD were observed in the nuclei. (B) Western blot of Zfp64, CSL and NICD in cytoplasmic and nuclear protein extracts. U2OS cells were transfected constructs encoding combinations of HA-tagged Zfp64, FLAG-tagged CSL, and V5-tagged NICD. Cytoplasmic and nuclear proteins were extracted separately, equal quantities of the proteins were separated by electrophoresis and blotted onto a membrane that was consecutively probed for each protein. (C) Nuclear:cytoplasmic ratios of the densitometric values of the western blot result shown in B.

• Supplemental Figure S3 (Adobe PDF) -

  Fig. S3. Northern blot analysis of Zfp64 expression in adult mouse organs.