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Figure 1


Fig. 1. Illustration of the protein-domain organization of Zfp64. The numbers denote zinc-finger motifs. Zinc-finger motifs 6-9 of isoform-D are highly homologous to the zinc-finger motifs 3-6 of the other isoforms, and are labeled 3', 4', 5', 6' (using the respective numbers). Zinc-finger motifs 5 and 10-13 of isoform-D are labeled 2', 7', 8', 9', 10'. The yeast-two-hybrid screening yielded the isoform-D-specific C-terminal fragment (Zfp-C'). A construct that consists of the region common to all the splicing variants (Zfp-N) and a construct that consists of the C-terminal-fragment specific for isoforms A, B and C (Zfp-C) were created and used in the yeast-two-hybrid assay.





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