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Figure 6


Fig. 6. Runx2 upregulates Zfp64 expression. (A) Luciferase activity assay. The Zfp64 promoter was activated by Runx2 in a dose-dependent manner. (B) MC3T3-E1, C2C12, and RD-C2 Runx2-deficient cell lines were transfected with AdGFP or AdRunx2. Zfp64 expression was measured by real-time RT-PCR, and shown as Runx2/GFP ratio (means ± s.e.). *P<0.05. (C) The distal ose2 element of the human Zfp64 promoter is crucial for its transactivation by Runx2. Deletions or mutations were introduced into the Zfp-luc reporter construct as illustrated, which was co-transfected with the mock or Runx2 expression plasmid into U2OS cells for a luciferase activity assay. (D) MC3T3-E1 cells or RD-C2 cells were treated with 100 ng/ml of human recombinant BMP2 protein. Expression of Runx2, Zfp64, Hey1 and Hes1 was examined by real-time RT-PCR and was expressed as fold-increase (mean ± s.e.) versus controls without BMP2. *P<0.05. ND, not detected.





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