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Fig. 3. (A) Effect of increasing CQ concentrations on the rate of DV alkalinisation (expressed as the inverse of the half-time) following H+ pump inhibition in CQS D10 (circles) and CQR 7G8 (squares) parasites. For D10 parasites, the experiment was performed both in the absence (
) and presence (
) of the H+ ionophore CCCP (100 nM). CQ and CCCP were added to saponin-isolated mature trophozoites 1 minute prior to the addition of the H+ pump inhibitor concanamycin A (100 nM). Data are averaged from three separate experiments for D10 and from four separate experiments for 7G8, and are shown as mean ± s.e.m. Where not shown, error bars fall within the symbol. (B) CQ-concentration-dependence of the initial rate of alkalinisation of the DV in CQR 7G8 parasites (estimated from the initial slope of the fluorescence traces) following inhibition of the H+ pump by concanamycin A (100 nM). CQ was added to saponin-isolated mature trophozoites 1 minute before the addition of concanamycin A. The fluorescence ratio was normalised between experiments by dividing by the maximum fluorescence ratio. The initial rate of alkalinisation in the absence of CQ was subtracted from the initial rates in the presence of CQ. The data (± s.e.m.) are averaged from four independent experiments; the line was drawn using a rectangular hyperbola, fitted to the data [y=ax/(b+x), where y is the initial rate of alkalinisation, x is the CQ concentration, a=0.06 second–1 and b=23 µM].
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