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Figure 5


Fig. 5. GFP-Mdv1p and -Caf4p partially localise to peroxisomes in a Fis1p-dependent manner. (A,D) caf4{Delta}/mdv1{Delta}/vps1{Delta} cells coexpressing GFP-Mdv1p (A) or GFP-Caf4p (D) and HcRed-PTS1 (per-Red). Top row: flattened z-stack showing faint colocalisation of GFP fusion with peroxisome (arrowheads). Bottom row: colocalisation is easier to detect in individual slices of the z-stack. (B,E) Mating experiment showing enhanced levels of Mdv1p and Caf4p on peroxisomes as a result of Fis1p-Pex15p expression. fis1{Delta}/vps1{Delta} cells expressing both Pex15p-Fis1p and either GFP-Mdv1p (B) or GFP-Caf4p (E) were mated with pex3{Delta} cells expressing HcRed-PTS1. Top row: flattened z-stack showing colocalisation with peroxisomes. Bottom row: colocalisation is easier to detect in individual slices of the z-stack. Peroxisome labelling with GFP is much stronger compared to A and D. (C,F) Both GFP fusions were mainly cytosolic when expressed in fis1{Delta} cells. Scale bar: 5 µm.





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