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Figure 3


Fig. 3. Fyn associates with both the full-length and the deletion mutant of {alpha}IIbβ3. (A,B) CHO cells stably expressing either full-length or truncated β3-subunits, together with the full-length {alpha}IIb subunit, were transiently transfected with the cDNAs encoding (A) GFP-Src or (B) GFP-Fyn. 48 hours after transfection, cells were re-plated on fibrinogen-coated plates for 1 hour. Cell extracts were subjected to immunoprecipitation using antibodies against the β3 subunit. The immunoprecipitates were then analyzed for (A) Src or (B) Fyn using anti-GFP antibodies. Expression levels of transfected integrins and GFP fusions are also shown.





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