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Figure 10


Fig. 10. PAI1 does not disrupt matrix adhesions. Cells were incubated overnight on coverslips in complete medium and then incubated for 3 hours in DMEM in the presence or absence of PAI1 (20 nM). Cells were subsequently fixed, and permeabilized. (A) Fibronectin and tensin were visualized by indirect immunofluorescence in untreated cells (panels a and b) and PAI1 treated cells (panels d and e). Panels c and e show the merged pictures. Yellow staining represents area of colocalization of tensin and fibronectin. Scale bar, 20 µm. (B) Paxillin and tensin were visualized by direct and indirect immunofluorescence in untreated cells (panels a and b) and PAI1 treated cells (panels d and e). Panels c and f shows merged pictures. Scale bar, 20 µm.





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