spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 11


Fig. 11. β5 knockdown inhibits PAI1 stimulation of matrix assembly. (A) Lysates from MG-63 cells transfected with either control siRNA or β5 siRNA were analyzed for β5 integrin and actin using western blotting. (B,C) MG-63 cells treated with either control or β5 siRNA were incubated with [125I]fibronectin for 6 hours in the presence of 20 nM PAI. Cells were extracted with 1% DOC, and [125I]fibronectin that had been incorporated into detergent-insoluble matrix was recovered by centrifugation and measured by {gamma}-scintillation. Experiments are representative of separate experiments performed in triplicate. Data are the mean ± s.e. of one experiment performed in triplicate. P<0.05 (n=3), t-test. All values were normalized to protein content. No significant difference in protein content was observed between control siRNA and β5 siRNA-treated cells.





Right arrow Return to article