QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 2. The PAI1-mediated increase in fibronectin matrix assembly does not require uPAR. (A) Cell lysates were prepared from MG-63 cells transfected with control siRNA or uPAR siRNA. uPAR and β5 integrin levels were analyzed by western blotting. The membrane was stripped and reprobed with anti-β-actin antibody as a loading control. (B) Cells layers from MG-63 cells transfected with control siRNA or uPAR siRNA were incubated with [¹²⁵I]fibronectin for 6 hours in the absence or presence of PAI1 (20 nM). Cell layers were extracted with 1% DOC, and [¹²⁵I]fibronectin that had been incorporated into the detergent-insoluble matrix was recovered by centrifugation and measured by -scintillation. Data are the mean ± s.e. of two experiments performed in duplicate. ^*P<0.05 (n=4), significantly different than untreated cells (t-test). (C) Cells layers from MG-63 cells transfected with control siRNA or uPAR siRNA were incubated with [¹²⁵I]fibronectin for 6 hours in the presence of the uPAR agonist P25 or the control peptide S25. Cell layers were washed and scraped into 1% DOC and cell-layer associated [¹²⁵I]fibronectin was measured by -scintillation. Data are the mean ± s.e. of two experiments performed in duplicate. ^*P<0.05 (n=4), significantly different than control cells (t-test).

Return to article