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Figure 6


Fig. 6. PAI1 disrupts focal adhesions. Monolayers of MG-63 cells were plated overnight onto coverslips in complete medium and then incubated for 3 hours with 20 nM of PAI1 or PAI1 mutants (PAI-1R and PAI-1 Q123K). Cells were subsequently fixed, permeabilized and stained for 1 hour with the clone 15F11 antibody against β5 integrin (panels a-d). After 1-hour staining using Alexa-Fluor-594-derivatized anti-mouse secondary antibody, cells were washed and labeled for an additional hour with a paxillin-FITC antibody (panels e-h). Panels i-l show merged images that indicate colocalization of β5 integrin with paxillin. Scale bar, 20 µm.





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