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Figure 7


Fig. 7. PAI1 disrupts actin stress fibers. MG-63 cells were plated overnight onto coverslips in complete medium and then incubated for 3 hours with 20 nM of PAI1 or PAI1 mutants (PAI-1R and PAI-1 Q123K). Cells were subsequently fixed, permeabilized and stained for 1 hour with the clone 15F11 antibody against β5 integrin. Cells were then washed and labeled for an additional hour using Alexa-Fluor-488-derivatized anti-mouse secondary antibody and Alexa-Fluor-594–phalloidin. Actin and β5 integrin were visualized by indirect immunofluorescence. Scale bar, 20 µm.





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