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Figure 7


Fig. 7. Fluorescence microscopy of F-actin distribution and caspase 3 activation in control and TNF{alpha}+CHX-treated TtT/GF cells, and wild-type and MHCIIB–/– embryonic fibroblasts. The cells were incubated with culture medium alone (control) or containing TNF{alpha}+CHX for 16 hours. Next, the cells were processed for fluorescence microscopy with Rhodamine-phalloidin and anti-active caspase 3. Control cells were flat and showed cytoplasmic extensions and cortical and cytoplasmic actin filaments. Control cells showed low levels of caspase 3 activation. Following TNF{alpha}+CHX treatment, the few TtT/GF cells and wild-type fibroblasts that remained attached were round and displayed caspase 3 activation. Some treated TtT/GF cells that remained flat showed no evidence of caspase 3 activation (arrows). Treated MHCIIB–/– cells that remained attached were flat and possessed actin fibers. Moreover, caspase 3 was active in these adherent, TNF{alpha}+CHX-treated MHCIIB–/– cells. Scale bar: 50 µm.





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