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Figure 8


Fig. 8. Overexpressed N-terminal portion of SUUR recruits HP1 to ectopic sites. Immunofluorescent staining of polytene chromosomes with antibodies against SUUR (green) and HP1 (red) after overexpression of the N-terminal SUUR fragment rs4 (A) and of the C-terminal SUUR fragment C17 (B) under the control of the Sgs3-GAL4 driver in the SuUR mutant background. Schematics of the rs4 and C17 constructs are depicted below the corresponding panels; the homology region to the SNF2 domain is in yellow, the homology region to the bromodomain is in brown, the negatively charged amino acid clusters are in blue, the positively charged amino acid clusters are in red, the NLSs are in grey and the HA-tag is in white.





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