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Fig. 1. Endocannabinoids trigger Ca²⁺ signaling and ER depletion in human endothelial cells. (A) Comparison of the effects of 100 µM histamine (n=18) and 10 µM anandamide (n=31) on cytosolic free-Ca²⁺ concentration in the presence of 2 mM extracellular Ca²⁺. (B) Cytoplasmic free-Ca²⁺ concentration upon stimulation with 100 µM histamine (n=19) and 10 µM anandamide (n=21) in the absence of extracellular Ca²⁺, followed by re-addition of 2 mM extracellular Ca²⁺ to reveal store-operated Ca²⁺ entry due to previous ER depletion. (C) Comparison of the effects of 100 µM histamine (n=4) and 10 µM anandamide (n=3) on free ER Ca²⁺ concentration in the absence of extracellular Ca²⁺, followed by re-addition of 2 mM extracellular Ca²⁺ to visualize ER Ca²⁺ refilling efficiency. (D) Concentration-response curves for anandamide (n=10) and metanandamide (n=10) on peak responses of cytosolic Ca²⁺ elevation in the nominal absence of extracellular Ca²⁺. Cytosolic (A,B,D) and ER (C) Ca²⁺ concentrations were recorded using fura-2 and YC4er, respectively. ^*P<0.01 versus histamine.

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