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Fig. 3. Anandamide/GPR55-induced Ca2+ signaling is linked to integrins. (A,B) The sensitivity of anandamide (10 µM) -triggered cytosolic Ca2+ signaling to various concentrations of extracellular Ca2+ (n=10), Ba2+ (n=24) and Sr2+ (n=31) was tested. (C) Comparison between the effect of 10 µM anandamide in Ca2+-containing buffer in the absence (n=20) or presence of 70 µM Mn2+ (n=40). (D) The effect of 10 µM anandamide on cytosolic free-Ca2+ concentration was monitored in the nominal Ca2+-free solution in the absence (n=25) or presence of the ROCK inhibitor Y27632 (10 µM, n=29). (E-G) The effects of antibodies (2 µM) against 
vβ3 integrin (LM609) (control, n=34; LM609, n=30) and the β1 (JB1A) (control, n=28; JB1A, n=33) and β3 subunits (B3A) (control, n=38; B3A, n=30) on cytosolic Ca2+ signaling induced by 10 µM anandamide was assessed in nominally Ca2+-free solution. (H) The effect of function-blocking β3-integrin antibody B3A on O1602 (10 µM) -initiated Ca2+ signaling in the presence of extracellular Ca2+ (control, n=65; B3A, n=84). Cytosolic free-Ca2+ concentrations were recorded using fura-2. *P<0.0001 versus the absence of the respective compound.
