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Figure 8


Fig. 8. NF{kappa}B is activated independently of anandamide-triggered Ca2+ signals. The effect of 10 µM anandamide on the (trans)location of GFP-tagged p65 subunit of NF{kappa}B was visualized in endothelial cells in buffer containing 2 mM Ca2+ in the absence (A) or presence (B) of 70 µM Mn2+. Images of endothelial cells that were transiently transfected with p65-GFP were taken 48 hours after transfection using a conventional fluorescence microscope. Representative images of at least 10 cells out of three experiments on alternate days are shown. Scale bars: 10 µm.





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