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Figure 2


Fig. 2. Characterisation of EML3 localisation by indirect immunofluorescence. Antibodies against human EML3 peptide sequences were used to detect the protein by immunoblot or immunofluorescence. (A) Immunoblots of total lysates of HeLa cells. P, preimmune serum; I, EML3 immune serum. (B) Localisation of EML3 in the different mitotic phases and in interphase of HeLa cells as indicated. Cells were fixed with methanol at –20°C and stained with serum against EML3 (middle left panels and red colour in merge), {alpha}-tubulin (middle right panels and green colour in merge) and with DAPI to visualise DNA (blue colour in merge). (C) EML3 was detected by indirect immunofluorescence after fixation with formaldehyde in interphase HeLa cells before and after addition of leptomycin B (LMB) to inhibit CRM1-dependent nuclear export. Scale bars: 10 µm.





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