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Figure 3


Fig. 3. Hrs-binding region in the cytoplasmic tail of IL-2Rβ. (A) Structure of wild-type IL-2Rβ and its deletion (d) mutants. The signal sequence, Flag tag, WSxWS motif and transmembrane region (TM) are indicated. (B) HEK293T cells were cotransfected with 2 µg Hrs and 2 µg wild-type IL-2Rβ or its mutants. Lysates of HEK293T cells (2x106) were immunoprecipitated with TU11 and immunoblotted with an anti-Hrs monoclonal antibody. The levels of IL-2Rβ and Hrs were examined by immunoblotting with an anti-Flag monoclonal antibody and anti-Hrs antibody, respectively. Total lysate: aliquots (1.25%) of lysates from the indicated cells (2x106) were immunoblotted with an anti-Hrs antibody or anti-Flag antibody. WT, wild-type; IP, immunoprecipitation; IB, immunoblotting.





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