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Fig. 8. Kinetics of the subcellular distribution of IL-2Rβ. (A) Kinetics of the IL-2Rβ subcellular localization. The indicated cells were grown on coverslips and treated as described in Fig. 5C. Fluorescence labeling was carried out for IL-2Rβ (red) and LAMP1 (green) in a or IL-2Rβ (green) and Hrs (red) in b. A large part of IL-2Rβ_d349-410 is not sorted to LAMP1-positive compartments (arrows) whereas some IL-2Rβ_d349-410 is delivered to LAMP1-positive compartments (arrowheads) at 120 minutes in MEFβ_d349-410 cells. Scale bars: 5 µm. (B) Degradation of IL-2Rβ in the transfectants. The radioactivity of culture supernatants (a), cell precipitate fractions (b) and TCA-soluble fractions of culture supernatants (c) was counted. ¹²⁵I-TU11 binding to parental MEF cells was 4.7% of that of MEFβ cells. The values represent the mean ± s.e.m. of triplicate determinations.

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