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Figure 3


Fig. 3. Dephospho-β-catenin colocalizes with p-LRP6, axin and APC at the plasma membrane in a Wnt-dependent (Kep1) or {Delta}N-LRP6-dependent (SK-BR-3) manner. Kep1 cells were induced with purified Wnt3A protein or vehicle for 2.5 hours (Kep1), or transfected with {Delta}N-LRP6-encoding plasmid (SK-BR-3) and analyzed for subcellular localization of dephospho-β-catenin and phospho-LRP6 (A,D), dephospho-β-catenin and axin (B), total β-catenin and APC (C), and dephospho-β-catenin and APC (E). Arrows denote plasma membrane localizations. Merged images are also shown, which include DAPI as a nuclear marker (A-C) or H2B-mRFP as a transfection marker (D,E).





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