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Fig. 1. Myosin II is enriched at the surface of 3D epithelial cysts. Caco-2 cells were embedded in Matrigel for 72 hours, fixed, and fluorescently double-labeled for F-actin (red) and either NMMIIA or NMMIIB (green). Cross-sections of Caco-2 cysts show that significant pools of NMMIIA and NMMIIB accumulate within F-actin bundles at the cyst surface (arrows). Scale bar: 20 µm.