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Figure 3


Fig. 3. The absence of Myosin 2 from furrow canals precipitates furrow regression in nulloX embryos. (A,B,D) Confocal images of PM furrows (Nrt, red) and Myosin 2 furrow canals (Myo-2, green) ingressing between adjacent nuclei in cellularizing embryos. (A) En face images from a single plane at the level of the furrow canals showing that in nulloX embryos, some furrows are missing (arrows) or have no Myosin 2 in the furrow canals (arrowheads). (B) Projected z-section from a nulloX embryo showing adjacent nuclei separated by: (1) a furrow with Myosin 2; (2) a furrow with no Myosin 2; or (3) a missing furrow. (C) The average percentage of disrupted furrows at progressive stages of cellularization in nulloX embryos grown at 23°C or 29°C (total disrupted furrows, blue; furrows with no Myosin 2, red; furrows missing, green). Each point represents 4-6 cellularizing embryos of the given furrow length, with ~200 interfaces scored per embryo. Error bars represent s.d. (D) Cross-sections at progressive phases of cellularization showing furrows with Myosin 2 between all nuclei in wild-type embryos. In nulloX embryos, some furrows are missing (arrows) or have no Myosin 2 in the furrow canals (arrowhead). (E) Kymographs showing furrow dynamics (GFP-Spider) in live cellularizing embryos (taken from supplementary material Movies 1 and 2). Three types of furrow dynamics are seen in nulloX embryos: (1) furrows that ingressed ~40 µm at rates comparable to those of the wild type; (2) furrows that ingressed to lengths >5 µm then regressed; and (3) furrows that ingressed to a length of only ~5 µm then regressed. Arrows follow the tip of the regressing furrows. (F) En face confocal image from a single plane at the level of the furrow canals showing PM furrows (Nrt, red) and Anillin furrow canals (green) in cellularizing nulloX embryos. Some furrows are missing (arrows) or have no Anillin in the furrow canals (arrowheads). Scale bars: 5 µm in A,B,D,F.





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