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Fig. 6. F-actin disruption mimics nulloX phenotypes. (A-E) Confocal images of PM furrows ingressing between adjacent nuclei in cellularizing wild-type embryos treated with Cyto-D. (A) En face images collected in a single plane at the level of the furrow canals showing that in embryos treated with a low dose of Cyto-D, some furrows (Dlg, red) are missing (arrows) or have no Myosin 2 (Myo-2, green) in the furrow canals (arrowheads). The furrow canal network is severely disrupted in embryos treated with higher doses of Cyto-D. (B) En face image collected in a single plane at the level of the furrow canals showing that in embryos treated with 1 µg/ml Cyto-D, some furrows (Nrt, red) are missing (arrows) or have no Anillin (green) in the furrow canals (arrowheads). (C) Cross-section from an embryo treated with 1 µg/ml Cyto-D showing Myosin 2 furrow canals (green) are absent from some furrows (Dlg, red; arrowheads). (D) Cross-section from an embryo treated with 1 µg/ml Cyto-D showing Dlg (red) spreading into some Myosin 2 furrow canals (green; arrowheads). (E) Cross-section from an embryo treated with 1 µg/ml Cyto-D showing some furrows (Nrt, red) are missing between adjacent nuclei (arrow) or have no Anillin (green) in the furrow canals (arrowhead). (F,G) Confocal images of PM furrows (Nrt, red) and basal junctions (Arm, green) in cellularizing wild-type embryos treated with 1 µg/ml Cyto-D. (F) Cross-sections showing basal junction defects, ranging from furrows that have some basal Arm accumulation (arrowhead) to those that have none (arrows). (G) En face images from a single plane at the level of the basal junctions showing basal junction defects, ranging from Arm plaques at some furrows (arrowheads) to diffuse Arm puncta at others (arrows). (H) En face images from a single plane, showing that Nullo (green) accumulates normally in furrow canals of Cyto-D-treated embryos. Scale bars: 5 µm.
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