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Figure 2


Fig. 2. UV-induced degradation of securin is not mediated by APC/C. (A) HeLa cells were transiently transfected with pSUPER-Cdh1 and pSUPER-Cdc20 or pSUPER alone, and harvested 48 hours after transfection. Where indicated, cells were irradiated 1 hour 30 minutes before harvesting. Extracts were Western blotted for the detection of CDH1, CDC20 and securin (hSec). Note that the western blot for CDC20 shows three bands, the upper two (and most prominent) of which are crossreactive and not specific to CDC20. (B) HeLa cells were transiently transfected with siRNA oligonucleotides against CDC27/APC3 or nonspecific control oligonucleotides (mock) and, after 48 hours, were irradiated or left untreated, and collected 1 hour 30 minutes later. Western blots of extracts were analyzed for CDC27/APC3, securin and {alpha}-tubulin levels. HeLa, lysates from nontransfected HeLa cells.





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