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Figure 4


Fig. 4. Amino acids 1-28 are sufficient to target proteins to droplets. AAM-B mutants were expressed in HeLa cells and processed for immunofluorescence. (A-C) Deletion of amino acids 29-61 in the juxtamembrane region (A) disrupted targeting to Bodipy 493/593-positive (B) droplets (C). (D-F) GFP was fused to the C-terminal end of AAM-B amino acids 1-28 and expressed in HeLa cells. The chimera (D) was found in an ER pattern with some protein localized to ADRP-marked (E) droplets (F). (G-I) AAM-B amino acids 1-38 fused to GFP (G) co-localized with ADRP (H) on droplets (I). (J-L) Deletion of amino acids 29-38 from full-length AAM-B (J) did not influence targeting to Bodipy-positive (K) droplets (L). Scale bars: 5 µm.





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