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Figure 8


Fig. 8. AAM-B on droplets can interact with itself. (A) Co-immunoprecipitation of Myc-tagged and HA-tagged AAM-B co-expressed in HeLa cells. HeLa cells were co-transfected with cDNA encoding either HA-tagged AAM-B, Myc-tagged AAM-B (lanes 2 and 4) or empty vector (lanes 1 and 3). The cells were solubilized with detergent and processed to immunoprecipitate AAM-B-Myc with {alpha}-Myc IgG. Samples of the cleared cell lysates (lanes 1 and 2) and the immunoprecipitates (lanes 3 and 4) were separated by SDS-PAGE and immunoblotted with {alpha}-HA IgG, {alpha}-Myc IgG or {alpha}-p63 IgG. (B-G) Wild-type AAM-B can recruit truncated AAM-B from the cytoplasm to droplets. HeLa cells were co-transfected with a cDNA encoding Myc-tagged N3 and an empty vector (B-D). Cells were grown for 15 hours, fixed and processed for immunofluorescence detection of HA (B) and Myc (C). HeLa cells were co-transfected with a cDNA encoding Myc-tagged N3 and HA-tagged wild-type AAM-B (E-G). Cells were grown for 15 hours, fixed and processed for immunofluorescence detection of HA (E) and Myc (F). Scale bars: 5 µm.





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