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First published online 13 May 2008
doi: 10.1242/jcs.028605
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Research Article |
1 Department of Molecular Biology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390-9148, USA
2 Department of Pathology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390-9148, USA
* Author for correspondence (e-mail: martin.kucej{at}utsouthwestern.edu)
Accepted 11 March 2008
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Summary |
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 Top Summary IntroductionResultsDiscussionMaterials and MethodsReferences |
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Key words: Abf2, Hsp60, Ilv5, Mitochondrial DNA, Mitochondrial nucleoid
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Introduction |
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). Histones are extensively modified, undergoing different post-translational modifications, depending on their chromosomal location (Saha et al., 2006). As a result, chromatin remodeling is an essential process in eukaryotic cells, having a crucial role in growth, development and epigenetic events.
The mitochondrial genomes are packaged with proteins in structures, which, by analogy to bacterial chromosomes, are called mitochondrial DNA (mtDNA) nucleoids (Chen and Butow, 2005; Kucej and Butow, 2007; Malka et al., 2006). In contrast to nuclear chromatin, there is less information on the organization and dynamics of mtDNA nucleoids and how these properties might affect mtDNA transactions, including gene expression and inheritance. Initial studies in yeast (Chen et al., 2005; Kaufman et al., 2000; Miyakawa et al., 1987), human (Bogenhagen et al., 2008; Cheng et al., 2005; Garrido et al., 2003; Wang and Bogenhagen, 2006), and frog (Bogenhagen et al., 2003) revealed that, in addition to DNA transaction factors, high-mobility group (HMG)-like proteins in addition to a set of chaperones and metabolic enzymes are the major nucleoid components. Mitochondrial nucleoids lack histones. Instead, many mitochondrial genomes of different organisms are putatively packaged by HMG-like proteins, such as Abf2 in the budding yeast Saccharomyces cerevisiae, and Tfam in animal cells (Chen and Butow, 2005; Kaufman et al., 2007; Malka et al., 2006). These proteins are able to bend and wrap dsDNA in vitro (Brewer et al., 2003; Fisher et al., 1992; Friddle et al., 2004). Abf2 readily associates with DNA without sequence specificity, although it has a decreased affinity for simple polyA sequences (Diffley and Stillman, 1992). This feature causes phased binding, which was found to occur in vitro on replication origins and promoter sequences (Diffley and Stillman, 1991; Fisher et al., 1992). The mtDNA in abf2 mutants is less protected and exhibits increased sensitivity to nuclease attack (Newman et al., 1996) and oxidative stress (O'Rourke et al., 2002). Nucleoids contain an assortment of proteins with no obvious role in mtDNA transactions. These `unexpected' nucleoid proteins, such as aconitase and Ilv5 in yeast, have turned out to be bifunctional with distinct metabolic activities and, as nucleoid proteins, novel activities with respect to their functions in the maintenance of mtDNA (Bateman et al., 2002b; Chen et al., 2007; Chen et al., 2005; Kaufman et al., 2003; Shadel, 2005; Zelenaya-Troitskaya et al., 1995).
Here, we demonstrate by chromatin immunoprecipitation (ChIP) that yeast Abf2 is a bona fide mtDNA-packaging protein. Owing to changes in mtDNA copy number, the ratio of Abf2 to mtDNA varies under different growth conditions, which is accompanied by variable sensitivity of mtDNA to micrococcal nuclease. In addition, other nucleoid proteins, such as Hsp60 and Ilv5, are recruited to nucleoids during glucose repression and amino-acid starvation, respectively. We propose that these protein localization dynamics constitute a mitochondrial nucleoid remodeling. Finally, the nucleoid remodeling seems to be essential for the maintenance of mtDNA, because some mutant forms of nucleoid proteins that destabilize mtDNA, such as the Hsp60 A144V mutant and the previously reported Ilv5 W327R mutant (Bateman et al., 2002a), have perturbed submitochondrial localization.
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Results |
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500-bp DNA fragments, and nucleoid complexes were immunoprecipitated using an anti-Myc antibody. The coimmunoprecipitated DNA was first used as a probe for Southern blot hybridization (Fig. 1A). ChIP DNA was labeled without amplification and hybridized to blots of total mtDNA digested with DraI. We found that the hybridization profile obtained with the ChIP DNA was similar to that obtained when CsCl-purified total mtDNA was used as a probe, indicating that Abf2p binds most regions of mtDNA. The difference in signal intensities between the strains expressing Abf2-13Myc and the untagged Abf2 demonstrates that ChIP with Abf2 was very efficient. As a result, almost all immunoprecipitated DNA from the Myc-tagged strain represented the DNA material that was specifically bound by Abf2. These observations strongly support the role of Abf2 as a major mtDNA-packaging protein.
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). However, the authors of that study examined mostly mitochondrial gene sequences. We wanted to compare representatives of all features on the yeast mtDNA, including intergenic regions. Therefore, we prepared DNA microarrays of 550-bp average length mtDNA fragments covering 70% of a wild-type mitochondrial genome without introns, which we used for mitochondrial ChIP-chip (Fig. 1D). Amplified DNA samples from Abf2-13Myc and untagged Abf2 ChIP experiments were labeled with Cy5; CsCl-purified, ultrasonically sheared total mtDNA that was amplified by the same procedure, was used as a Cy3-labeled control (supplementary material Fig. S1). Microarray hybridization datasets of Abf2-13Myc experimental samples and Abf2 controls (supplementary material Fig. S3) were compared using a median-percentile rank method, as described previously (Buck and Lieb, 2004). When DNA spots with the same median-percentile ranks were grouped, a small subset of sequences appeared as a second peak (Fig. 1B). This group of sequences was represented almost exclusively by long, complex, GC rich sequences located in COX1, COX3, COB, ATP6, ATP9, 15S_rRNA and 21S_rRNA gene loci (Fig. 1C,D). Normally, only these enriched sequences would be considered to be bound by an analyzed protein. However, since the amount of crosslinked DNA was much higher in Abf2-13Myc samples compared with Abf2 controls (Fig. 1A), the correct interpretation is that Abf2 protein binds most of the mtDNA sequences with a relatively higher affinity for GC-rich gene regions.
Abf2 protein-protein interactions in mitochondria
We performed ChIP-on-chip (ChIP-chip) assays with two additional mitochondrial nucleoid proteins, namely Aco1 in glycerol medium and Ilv5 in medium without amino acids. No specific DNA enrichment was observed in these experiments. The most probable cause is very inefficient crosslinking of the examined proteins to mtDNA. When an excessive treatment by formaldehyde was applied, mitochondrial chromatin became resistant to DNA fragmentation and thus unusable for ChIP. It has been indeed observed that Aco1, for instance, exhibits only a weak binding to dsDNA (Chen et al., 2007). Also, it is conceivable that some nucleoid proteins are preferentially retained in proximity of mtDNA by protein-protein interactions with other DNA-binding proteins. We therefore asked whether such proteins can be coimmunoprecipitated with an mtDNA-binding protein, namely Abf2 (Fig. 2). Mitochondria isolated from yeast strains that express Abf2-13Myc and Abf2 as a control were crosslinked with dithiobis(succinimidylpropionate) (DSP) and lysed with a non-ionic detergent. The lysate was treated with DNaseI to prevent detection of protein interactions mediated by DNA (Fig. 2C). Abf2-13Myc protein was immunoprecipitated from the lysate using an anti-Myc antibody. The crosslinks were reversed and coimmunoprecipitated proteins were analyzed by gel electrophoresis (Fig. 2B). The examination of silver-stained bands, specific to the sample from the Abf2-13Myc expressing strain, revealed Aco1, Ald4, Idh1, Idh2 and Kgd1 as the major interacting proteins of Abf2. The proteins identified in this screen have been previously found in purified formaldehyde-fixed nucleoids (Kaufman et al., 2000). Thus, protein crosslinking with DSP further supports the localization of these proteins in nucleoids. Moreover, these proteins directly interact or are in proximity to the major nucleoid protein Abf2.
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Remodeling of mitochondrial nucleoids
Although the composition of mitochondrial nucleoids in yeast has been reasonably well-characterized, there is little information on whether nucleoids undergo structural or compositional remodeling. We therefore examined these issues in cells in which the respiration was repressed by glucose and activated by growth of cells on glycerol. To investigate whether nucleoid remodeling occurs under these conditions, we assessed the sensitivity of selected mtDNA sequences to micrococcal-nuclease digestion in toluene-permeabilized mitochondria (Newman et al., 1996). Nucleoids that are in a more compact structure or more covered with proteins should be more resistant to nuclease digestion, whereas nucleoids with a more open structure should be more sensitive to nuclease digestion. Accordingly, we compared three test regions of the mitochondrial genome – the 21S_rRNA region 71, the ATP6 region 33 and the intergenic DNA region 81 (supplementary material Table S1, Fig. 1D) – for their sensitivity to nuclease digestion. We found that these sequences were more resistant to nuclease attack in mitochondria isolated from glucose-grown cells than from glycerol-grown cells (Fig. 3A-C). Thus, the structure of mitochondrial nucleoids differs in these conditions and is more open in glycerol-grown cells compared with glucose-grown cells.
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). Thus, the nucleoid remodeling observed by micrococcal-nuclease-sensitivity assays could be accomplished by modulation of Abf2 levels, for example, by increasing the amount of Abf2 in glucose medium, or decreasing it in glycerol medium. However, expression of Abf2 was comparable in BY4741 cells grown in both types of medium (Fig. 3D). Notably, mtDNA copy number can vary too, depending on whether cells grow in glucose or on a non-fermentable carbon source (Goldthwaite et al., 1974), providing another means for nucleoid remodeling. Therefore, we measured mtDNA abundance in the BY4741 strain used in this study, and confirmed that mtDNA copy number was twofold higher in glycerol-grown cells compared with glucose-grown cells (Fig. 3E). As a result, the Abf2 to mtDNA ratio in whole cells is decreased by a factor of two in glycerol medium. In agreement with this observation, nucleoids in glycerol-grown cells were less protected from nuclease digestion.
To examine whether the distribution of Abf2 to nucleoids varies under these different metabolic conditions, we assessed the submitochondrial localization of Abf2 by sedimentation of mitochondrial lysates in sucrose gradients. Purified mitochondria were lysed with a nonionic detergent and loaded onto sucrose gradients (20%-60%-80%). MtDNA was found in fractions near the bottom of the gradient (Fig. 4B), whereas the mitochondrial matrix marker protein Mdh1 remained near the top (Fig. 4A). We repeatedly observed that under our experimental conditions, Abf2 largely cofractionated with mtDNA in cells grown in both glucose and glycerol medium (Fig. 4A,B). We quantified the amount of mtDNA and Abf2 in the first seven fractions from the bottom, and confirmed that the average Abf2-to-mtDNA ratio in glycerol-grown cells reaches 59±21% of the ratio in glucose-grown cells. This reflects changes in the whole-cell ratio of Abf2 to mtDNA (Fig. 3D,E). In summary, because the level of expression and the pattern of distribution of Abf2 between nucleoids and the matrix does not vary – whereas the copy number of mtDNA does – the amount of Abf2 associated with mtDNA effectively decreases in mitochondrial nucleoids of glycerol-grown cells. This provides an explanation for a more-open structure of nucleoids detected by micrococcal nuclease.
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We have previously shown that Ilv5 is required for parsing mtDNA into a greater number of nucleoids during amino-acid starvation (MacAlpine et al., 2000). Therefore, we compared the distribution of Ilv5 and that of other nucleoid proteins in sucrose gradients of mitochondrial extracts from cells grown in minimal medium with no amino acids, or with isoleucine, leucine, valine as a control (Fig. 4C,D). We repeatedly found that there was more Ilv5 present in mtDNA-containing fractions of mitochondria that were isolated from cells that had been starved for amino acids compared with those isolated from control cells. At the same time, the distribution of other proteins to nucleoids was unchanged (or slightly lower, which could be attributed to decreased expression levels of these proteins under conditions of amino-acid starvation). These results suggest underlying functional differences for these nucleoid proteins that are reflected in their different responses to growth conditions. Together, these experiments demonstrate that mitochondrial nucleoids undergo specific compositional remodeling in response to various metabolic cues.
Mutant forms of bifunctional Hsp60 and Ilv5 proteins that cause mtDNA instability despite their `conventional' functions being preserved have been characterized (Bateman et al., 2002b; Kaufman et al., 2003). Molecular functions of Hsp60 and Ilv5 proteins relating to the maintenance of mtDNA are not yet understood, but it has been demonstrated that, in certain ilv5 mutants, which exhibit an mtDNA-instability phenotype, the submitochondrial localization of Ilv5 is altered; namely, aggregates of mutant Ilv5 protein are formed in the mitochondrial matrix (Bateman et al., 2002a). We investigated whether the A144V mutant form of Hsp60, which is unable to maintain mtDNA, also has impaired submitochondrial localization. We compared the distribution of Hsp60 A144V with the wild-type protein in sucrose gradients of mitochondrial lysates (Fig. 5). The levels of the A144V protein that we found associated with mtDNA were substantially elevated compared with that of the wild-type protein both in glucose and glycerol growth media. Thus, Hsp60 mutant protein, which causes defects in mtDNA metabolism, exhibits an altered recruitment to nucleoids.
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Discussion |
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First, using mitochondrial ChIP-chip assay, we demonstrated that Abf2 binds most of the mitochondrial genome and is, therefore, a bona fide mtDNA-packaging protein (Fig. 1). Thus, our study supports the hypothesis (that originated from previous in vitro studies (Brewer et al., 2003; Friddle et al., 2004), that the abundance of Abf2 in nucleoids determines the degree of compaction and the overall structure of nucleoids. In addition, our ChIP-chip experiments revealed a preferential binding of Abf2 to complex GC-rich sequences in vivo. In essence, the yeast mtDNA consists of gene sequences, which are the only complex GC-rich features on the genome, plus AT-rich spacers that are often interrupted by short GC clusters. Thus, the Abf2 distribution on the mitochondrial genome might reflect the inability of Abf2 to bind oligoA sequences, a fact that has been demonstrated previously in vitro (Diffley and Stillman, 1992). However, it is conceivable that the observed binding pattern represents an intentional recruitment of Abf2 to gene sequences to ensure better protection, packaging or other regulatory functions.
Second, we show that, because of a twofold increase in mtDNA copy number and stable expression of Abf2, the average whole-cell ratio of Abf2-to-mtDNA is decreased in cells grown in glycerol-containing medium compared with cells grown in medium containing glucose (Fig. 3D,E). Notably, the distribution of Abf2 between nucleoids and the matrix remains similar both in glucose-enriched and glycerol-enriched medium under our experimental conditions (Fig. 4A). Therefore, we conclude that, in transcriptionally active nucleoids, there is effectively less Abf2 bound to each mtDNA molecule. Our comparison of nuclease sensitivity of nucleoids in mitochondria isolated from glucose- and glycerol-grown cells supports this hypothesis, because glucose nucleoids are more resistant to micrococcal nuclease than glycerol nucleoids (Fig. 3A-C). The functional significance of this remodeling still remains unknown. It is conceivable that Abf2 regulates the access of other proteins to mtDNA. To test this, we examined a mitochondrial RNA polymerase, Rpo41, but found that it is predominantly associated with nucleoids, both in glucose- and glycerol-containing medium (M.K., unpublished data). Although the access of Rpo41 to mtDNA does not seem to be regulated by Abf2 there might be other DNA-binding factors that are affected by the interaction of Abf2 with mtDNA.
Although Abf2 is the major mtDNA-binding protein, other nucleoid proteins are also likely to contribute to the overall nucleoid structure and DNA protection under various growth conditions. Indeed, we show that the distribution of some nucleoid proteins is connected to metabolic cues; Hsp60 and Ilv5 are recruited to nucleoids during glucose repression and amino-acid starvation, respectively (Fig. 4). It is known that Ilv5 is required to parse DNA into greater number of nucleoids in medium lacking amino acids (MacAlpine et al., 2000), however, the role for Hsp60 during glucose repression is less clear. Hsp60 has been proposed to function in nucleoid division (Kaufman et al., 2003). It is conceivable that the recruitment of Hsp60 to nucleoids in glucose-grown cells reflects an increased requirement for this protein in order to ensure the reliable propagation of mitochondrial nucleoids under conditions in which loss of mtDNA is not lethal.
Mutants of Ilv5 that exhibit an mtDNA-instability phenotype have been previously studied (Bateman et al., 2002a). Some of these mutant Ilv5 proteins cannot perform their function in nucleoids because they form protein aggregates in the mitochondrial matrix. We thought that Hsp60 A144V mutant protein (Kaufman et al., 2003), which causes mtDNA instability, might be inadequately localized in the mitochondrial nucleoids as well. Surprisingly, we observed that Hsp60 A144V mutant proteins are associated with mtDNA in a great surplus (Fig. 5). Thus, the mtDNA-instability phenotype of the Hsp60 A144V mutant might be caused by this excessive presence of Hsp60 in the mitochondrial nucleoids. These two examples of an altered localization of nucleoid proteins suggest that correct recruitment of nucleoid factors and remodeling of mitochondrial nucleoprotein is essential for the maintenance of mtDNA under various metabolic conditions.
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Materials and Methods |
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). A wild-type untagged strain was used as a negative control throughout the ChIP-chip experiments. Mitochondria were isolated from cells grown in 2L YPG medium (1% yeast extract, 2% peptone, 3% glycerol) and purified on HistodenzTM (Sigma) gradients (Diekert et al., 2001). Approximately 2 mg of mitochondria were crosslinked with 1% formaldehyde for 2 hours at 4°C in 2 ml of 0.5M sucrose, 20 mM HEPES pH 7.4, 2 mM EDTA, 7 mM β-mercaptoethanol. After crosslinking, the mitochondria were lysed for 5 minutes at 4°C in 0.5% Nonidet P40 in the presence of protease inhibitor mix CompleteTM (Roche) and 50 mM NaCl. The lysed mitochondria were sheared using ultrasound for 10x25 seconds with a Branson Sonifier 450 (output 20%, setting 3). An aliquot of the sheared DNA was purified and examined by agarose gel electrophoresis. The size of the resulting DNA fragments ranged from about 400 bp to 2 kbp. Prior to immunoprecipitation the samples were precleared with Protein G agarose (Roche). The extracts were incubated overnight at 4°C with 2 µg of mouse antibodies against the Myc epitope (Roche). The immunocomplexes were precipitated with Protein G agarose (Roche) as specified by the manufacturer, except that 2 mM EDTA was present during all washing steps. The crosslinked DNA was released from the beads by incubating the samples at 65°C overnight in 1% SDS, 50 mM Tris-HCl pH 8.0, and 2 mM EDTA. The DNA samples were purified with Qiaquick® PCR-purification kit and amplified using a linear amplification method (Liu et al., 2003). Briefly, the DNA was dephosphorylated and dT-tailed with terminal transferase. T7 RNA polymerase promoters were incorporated into the extremities of DNA fragments and RNA was synthesized in vitro using the MEGAscript® T7 kit (Ambion). Approximately 0.5-1 µg of synthesized RNA was labeled using the AminoAllyl cDNA-labeling kit (Ambion). Cy5 mono NHS ester (Amersham) was used to label the cDNA sample from Abf2p-13Myc and the untagged strains. Cy3 mono NHS ester was conjugated with cDNA amplified identically from CsCl-purified mtDNA from the intronless MCC109 strain. The Cy5 and Cy3 labeled samples were mixed and used for hybridizations with microarrays. Custom microarrays were printed with PCR-amplified regions of intronless mtDNA by the University of Texas Southwestern Medical Center Microarray Core Facility (supplementary material Table S1). Since S. cerevisiae mtDNA contains many variable regions, some sequences were amplified from mtDNA of the FY1679 strain, which has been used to determine the complete mtDNA sequence (Foury et al., 1998). For ori5 and VAR1 regions, HS40 that contained the entire ori5 region, and VAR1 petite mtDNAs were used, respectively. In total, about 70% of the genome was covered with representatives of all features: ORFs, rRNAs, tRNA genes, promoters, oris, GC clusters, and AT rich spacer regions. Sequences of all oligonucleotides used for PCR reactions can be found in supplementary material Table S1. The ChIP-chip experiments were repeated three times with the Abf2-13Myc expressing strain and four times with the wild-type strain (supplementary material Fig. S3). The datasets were analyzed by a median-percentile ranking method as previously described (Buck and Lieb, 2004).
Nuclease-sensitivity assay
Nuclease sensitivity of mitochondrial nucleoids in organello was assessed as previously described with modifications (Newman et al., 1996). Mitochondria were isolated from 3 l YPD medium (1% yeast extract, 2% peptone, 5% glucose) or 2 l YPG cultures of the strain BY4741 and were further purified on HistodenzTM (Sigma) gradients. Approximately 1 mg of mitochondria was treated with 1% toluene for 15 min at 22°C in 1 ml of 0.5 M sucrose, 20 mM Tris-HCl pH 7.4, 2 mM EDTA. The permeable mitochondria were collected by centrifugation and treated with 0.2 U/ml micrococcal nuclease (USB) at 30°C in 0.5 M sucrose, 20 mM Tris-HCl pH 7.4, 2 mM EDTA and 4 mM CaCl2. Without the addition of micrococcal nuclease, mtDNA was stable for at least 60 min in the presence of 4 mM CaCl2 (supplementary material Fig. S4A). The reactions were stopped with 10 mM EGTA pH 8.4. To isolate the DNA, mitochondria were treated for 2 hours at 65°C with 0.2 mg/ml proteinase K in the presence of 1% SDS. The DNA was extracted with phenol and precipitated with isopropanol in the presence of 5 µg of linear acrylamide (Ambion). The DNA was digested with HaeIII, separated in agarose gels and transferred to nylon membranes. The blots were hybridized at 62°C in 6xSSC, 0.5% SDS and 5xDenhardt solution with various probes PCR-amplified from mtDNA (21S_rRNA/71, ATP6/33, product 81, supplementary material Table S1) labeled with [
-32P]dATP (Perkin Elmer) using the Random Primed DNA Labeling Kit (Roche). Blots were washed twice for 30 minutes with 6xSSC, 0.5% SDS at 30°C and twice for 30 minutes with 0.1xSSC, 0.5% SDS at 62°C. The signal intensities were quantified using a phosphorImager and ImageQuant software (Molecular Dynamics).
Submitochondrial fractionation
The mitochondria were isolated from 3-l YPD (5% glucose) and 2-l YPG cultures of the BY4741 strain grown at 30°C, 3-l YPD (5% glucose) and 2-l YPG cultures of W303-1B 
hsp60::HIS3 [pRS415-HSP60] and W303-1B hsp60::HIS3 [pRS415-hsp60-A144V] (Kaufman et al., 2003) grown at 26°C, and 3-l cultures of the MCC109 strain grown in SD (5% glucose) with or without the addition of 0.5 g/l isoleucine, leucine, and valine. The gradient-purified mitochondria (1 mg/ml) were lysed in 0.5% Nonidet P40 in 0.5 M sucrose, 20 mM Tris-HCl pH 7.4, 2 mM EDTA, 50 mM NaCl, 7 mM β-mercaptoethanol, and 1xCompleteTM (Roche) for 5 minutes on ice. The same amount of lysed mitochondria (1 mg of mitochondrial proteins) was loaded on top of a stepwise gradient composed of 4 ml of 20%, 2 ml of 60%, and 1 ml of 80% sucrose solution in 20 mM Tris-HCl pH 7.4, 2 mM EDTA, 50 mM NaCl, and 7 mM β-mercaptoethanol. After centrifugation at 110,000 g for 70 minutes at 4°C, 0.5-ml fractions were collected from the bottom of the tubes. The fractions were analyzed for the presence of mtDNA by Southern dot blot hybridizations as follows: 50 µl from each fraction were treated overnight with 20 µg proteinase K (USB) and denatured in 0.1 M NaOH for 20 min at 37°C. The DNA was neutralized in 6xSSC and loaded onto nylon membranes using a multi-well manifold blotter (Bio-Rad). The membranes were hybridized at 62°C in 6xSSC, 0.5% SDS, 5xDenhardt solution with ATP6/33 PCR product, labeled with P32-dATP (Perkin Elmer) using the Random Primed DNA Labeling Kit (Roche). The dot blots were washed twice for 30 minutes with 6xSSC, 0.5% SDS at 30°C and twice for 30 minutes with 0.1xSSC, 0.5% SDS at 62°C. For protein analysis, 12-µl aliquots from each fraction were separated in 4%-20% gradient polyacrylamide gels (Bio-Rad), blotted to nitrocellulose membranes, and probed with rabbit polyclonal antibodies against Abf2, Ilv5, Hsp60, Aco1, and Mdh1 and with goat anti-rabbit secondary antibodies conjugated with horseradish peroxidase (Bio-Rad). The immunocomplexes were detected by chemiluminescence with ECLTM reagents (Amersham). To minimize chemiluminescence variations, membranes were always processed at the same time in one solution and exposed side by side on a single film. Also, a single nitrocellulose membrane, not parallel membranes, was used to detect all proteins. All gradients and western blots were repeated, yielding almost identical results.
Mitochondrial DNA copy number
The BY4741 cells were grown in 100 ml of YPD and YPG medium. The total DNA was isolated by phenol/glass bead treatment. MtDNA copy number was measured by hybridizations of blots containing HaeIII-digested total cellular DNA. The membranes were hybridized with 21S_rRNA probe 71 and ACT1 probe 117 (supplementary material Table S1). Hybridization, washing and detection of DNA blots was carried out as described elsewhere in Materials and Methods. Band intensities were quantified using a phosphorImager (Molecular Dynamics) and ImageQuant 5.0 software (Molecular Dynamics).
Abf2 protein interactions
Pure mitochondria (1 mg) from YPG-grown MCC109 and MCC109 Abf2-13Myc strains were suspended in 0.6 M sorbitol, 40 mM HEPES-KOH pH 7.4 to obtain a total volume of 1 ml. Mitochondria were incubated for 2 hours on ice with or without 1 mM DSP. To stop the crosslinking reaction, 2.5 M glycine was added to the final concentration of 100 mM. Mitochondria were isolated by centrifugation at 4°C, 20,000 g for 30 minutes, resuspended in 0.5 M sucrose, 50 mM Tris-HCl pH 7.4, 0.05 M NaCl, 0.5% Nonidet P40, 1xCompleteTM, and incubated on ice for 15 minutes. To digest the mtDNA, 100U of DNaseI and MgCl2 to a concentration of 25 mM was added and the suspension was incubated at 30°C for 1 hour. The insoluble material was removed by centrifugation at 20,000 g for 10 minutes. About 15 µg of anti-Myc antibody was added and the samples were incubated on a rocking platform at 4°C for 2 hours, followed by incubation with a 100 µl of Protein G-Agarose (Roche) at 4°C for 16 hours. The agarose beads were washed twice in 50 mM Tris-HCl pH 7.4, 0.5 M NaCl, 1% Nonidet P40, 1xCompleteTM, twice in 50 mM Tris-HCl pH 7.4, 0.5 M NaCl, 0.1% Nonidet P40, and once in 50 mM Tris-HCl pH 7.4, 0.1% Nonidet P40. To reverse the crosslinks, the beads were suspended in 160 µl of 60 mM Tris-HCl pH 6.8, 2% SDS, 10% glycerol, 5% β-mercaptoethanol, 0.25% Bromophenol Blue and incubated at 92°C for 15 minutes. The proteins separated by SDS-PAGE visualized using silver staining. Bands of interest were cut out and analyzed by the Protein Chemistry Technology Center of Southwestern Medical Center at Dallas. Briefly, the proteins were in-gel digested by modified porcine trypsin, the peptide extracts were injected into a nano-HPLC–MS-MS system, and mass spectrometric data were searched against the NCBI non-redundant protein sequence database.
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Acknowledgments |
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Footnotes |
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Present address: Department of Developmental Biology, University of Texas Southwestern Medical Center, 6000 Harry Hines Boulevard, Dallas, TX 75390-9148, USA 
Present address: Department of Biochemistry and Molecular Biology, SUNY Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA
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References |
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) and glycerol-containing (
) medium. Representative autoradiograms are shown in 
