JCS028605 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 -

  Adobe PDF

• Supplemental Figure S1 -

  Fig. S1. Strategy for genomic mapping of Abf2p mtDNA binding sites by mitochondrial ChIP-chip analysis. The detailed description is in the Materials and Methods (Mitochondrial ChIP-chip assay).

• Supplemental Figure S2 -

  Fig. S2. Western blot of protein extracts from BY4741 strains expressing Abf2-13Myc and untagged Abf2 proteins detected using an anti-Myc antibody.

• Supplemental Figure S3 -

  Fig. S3. Plots of the Cy5 and Cy3 values from the microarray hybridization experiments as described in Fig. S1.

• Supplemental Figure S4 -

  Fig. S4. Nuclease-sensitivity assays. (A) MtDNA was stable in Ca²⁺ buffer. Toluene-treated mitochondria were incubated in 4 mM CaCl₂ with or without the addition of 0.2 U/ml micrococcal nuclease for 60 minutes. DNA was isolated, separated by agarose-gel electrophoresis and blotted to nylon membranes. Blots were hybridized with a PCR probe prepared from 21S_rRNA gene. (B) Mitochondria rendered permeable by toluene were treated with micrococcal nuclease for the indicated time intervals (0-15 minutes). The DNA was isolated, digested with HaeIII and transferred to nylon membranes, which were hybridized with probes derived from the indicated regions of mtDNA.