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Figure 4


Fig. 4. Distribution of nucleoid proteins in sucrose gradients. Sucrose gradients of an equal amount of lysed purified mitochondria (~1 mg of proteins) isolated from BY4741 cells grown in glucose and glycerol containing medium (A,B) and MCC109 cells grown in SD medium with isoleucine, leucine, valine or no amino acids (C,D) were collected in 16 fractions. Every fraction was probed for the presence of proteins Abf2, Mdh1, Hsp60, Aco1, Ilv5 (A,C) and mtDNA (B,D). Mdh1 is a matrix protein, which represents the control for the total lysis of mitochondria and the presence of matrix contamination. The combined occurrence of Abf2 and mtDNA indicates gradient fractions, which contain nucleoids. (A) Hsp60 is the only protein increased in fractions containing nucleoids, when cells are grown in glucose medium compared with glycerol medium. (C) Ilv5 is the only protein increased in fractions containing nucleoids, when cells are grown in medium without amino acids compared with medium containing isoleucine, leucine and valine.





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