JCS026989 Supplementary Material

Files in this Data Supplement:

• Supplemental Table S1 -

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• Supplemental Table S2 -

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• Supplemental Figure S1 -

  Fig. S1. Quantitative evaluation of radial nuclear clone probe distributions using 3RRD software (n=number of nuclei) for (A) human fibroblasts and tumor cell lines, (B) Mel Juso and (C) SW620.

• Supplemental Figure S2 -

  Fig. S2. Overview of evolutionary chromosome rearrangements in great apes and gibbons (small apes) concerning (A) human chromosome 2, (B) human chromosomes 5 and 17 and (C) human chromosome 7. To the right of each chromosome, the mapping position of large-insert clones used in this study is given.

• Supplemental Figure S3 -

  Fig. S3. Selected metaphase FISH experiments, illustrating evolutionary and tumor-related rearrangements involving chromosomes 5 and 17, respectively. (A) FISH to gorilla chromosomes with pooled large-insert clones representing chromosome 17 early-replicating (green) and chromosome 5 late-replicating (red) loci. (B) Chromosome 17 painting experiment to a Mel Juso metaphase. (C) Chromosome 5 (green) and 20 (red) painting to a SW620 metaphase. (D) Chromosome 5p (green) and 7p (red) painting to a SW620 metaphase. (E-K) Quantitative evaluation of radial probe distributions in the interphase nucleus or the respective chromosome territory, using 3D-RRD or eADS software, respectively (n=number of nuclei).

• Supplemental Figure S4 -

  Fig. S4. (A-D) Selected metaphase FISH experiments, illustrating evolutionary and tumor-related rearrangements involving chromosome 7. (A) Human and (B) gorilla metaphases after FISH with human chromosome-7-specific pools of large-insert clones representing early-replicating (green) and late-replicating (red) loci. (C,D) Chromosome 7p (green) and 7q (red) arm painting experiments to (C) Karpas 384 and (D) Mel Juso metaphases. (E-J) Quantitative evaluation of radial probe distributions in the interphase nucleus or the respective chromosome territory, using 3D-RRD or eADS software (n=number of nuclei).

• Supplemental Figure S5 -

  Fig. S5. Graphic display of correlations between local gene density, isochore GC content and replication timing (A) of all 64 large-insert clones used in this study and (B-E) of subsets of pooled clones used as FISH probes in the various 3D-FISH experiments.