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Figure 1


Fig. 1. (A-D) Representative FISH experiments with a two-color clone pool for one early-replicating (green) and/or late-replicating (red) locus per chromosome 1-22 and X. (A) Human metaphase chromosomes. (B-G) Dual-color displays of a representative human fibroblast nucleus (maximum intensity projection) after 3D-FISH of the chromosomes 1-22;X clone pools and double-pulse-labeled replication foci. Scale bar: 5 µm. (B) Early-replication foci in green, late-replication foci in red. (C) Early-replicating clone FISH signals in green, late-replicating clone FISH signals in red. (D) Early-replicating clone FISH signals in green, early-replication foci in red. (E) Late-replicating clone FISH signals in green, early-replication foci in red. (F) Early-replicating clone FISH signals in green, late-replication foci in red. (G) Late-replicating clone FISH signals in green, late-replication foci in red. (H) 3D reconstructed human fibroblast nucleus. (I,J) 3D maximum intensity projections of nuclei from hybridized tumor cell lines Mel Juso and SW620. DAPI counterstain in blue. Scale bar: 5 µm. (K-M) Quantitative evaluation of the radial probe distribution in the nucleus (n=number of nuclei) using eADS software for (K) human fibroblasts, together with early-replication and late-replication foci, (L) Mel Juso and (M) SW620.





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