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Figure 4


Fig. 4. Localization of the Nesprin-2 C-terminal isoform is affected in KO epidermis. (A-F) WT (A-C) and KO (D-F) frozen skin sections were stained with the N- and C-terminally directed anti-Nesprin-2 antibodies K56-386 and pAbK1. pAbK1 stains the NE in WT (B, arrowheads in inset), which is rarely apparent in the KO epidermis (E, arrowhead in inset). Hf, hair follicle; de, dermis; epi, epidermis. The broken line delineates the location of the basement membrane. Scale bars: 10 µm. (G) Nesprin-2-Giant–K56-386 immunocomplexes from mouse keratinocytes (PAM212) were probed (WB) with pAbK1 and K56-386. Arrowheads, small C-terminal Nesprin-2 isoforms; arrows, Nesprin-2-Giant isoform. Detection of the 800-kDa band in the K56-386 immunoblot is rarely possible in the input, but clearly visible in the immunoprecipitation lane. For the pAbK1 immunoblot, the sample was run in parallel on a separate gel. Silver staining is used as control (HC: IgG heavy chain).





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