QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 8. Nesprin-2 Giant affects nuclear morphology and size in the epidermis. (A-F) WT (A-C) and KO (D-F) frozen skin sections were stained with emerin-specific antibodies as indicated in the lower left of each frame. Note the increased nuclear size of KO epithelial cells. Emerin staining is NE-restricted in WT dermal cells (arrowheads, A-C), whereas, in the KO dermis (D,E and arrows in F), an abnormal staining pattern is evident. (G-L) Primary KO (G-I) and WT (J-L) keratinocytes were subjected to indirect immunofluorescence using anti-Nesprin-2 antibodies as indicated. K56-386-negative cells have an abnormal pAbK1 staining and misshapen nuclei (arrows, H,I). Cells exhibiting weak K56-386 staining (asterisks, G) have a normal nucleus and have pAbK1 staining at the NE (arrowheads, H). Note that the K56-386 and pAbK1 stainings are restricted to the NE in WT cells (K). Insets in J-L are higher-magnifications from another area of the same cover slip. Insets in A,D,I are higher-magnifications of the respective boxed areas in B,E,I, respectively. Confocal images are shown. epi, epidermis; de, dermis; hf, hair follicle. Scale bars: 10 µm.

Return to article