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Figure 3


Fig. 3. Transmission electron microscopy of 4 dpf wild-type, IFT57 and IFT88 mutant retinas. (A-C) Low-magnification electron micrographs of wild-type and mutant retinas, illustrating the outer segment (OS), inner segment (IS) and nucleus (N). Wild type and IFT57 mutants both exhibit photoreceptor outer segments, but IFT88 mutants lack these structures. (D,E) High-magnification micrographs of wild-type and IFT57 mutant retinas. Wild-type photoreceptors exhibit well-organized disk stacking within the outer segment, and the connecting cilium (black arrow) is observable. IFT57 mutants also demonstrate normal disk stacking within the outer segment, and the base of the connecting cilium (black arrow) is evident. An accumulation of vesicles (black arrowheads) is observed at the base of the cilium. Asterisk indicates centrioles oriented at right angles within the photoreceptor. (F-H) Immunogold labeling of rhodopsin in wild-type retinas shows dense labeling within the outer segment. Outer segments are outlined by broken lines and insets are magnified views of the boxed area in each panel, which illustrate gold particle density. IFT57 mutant outer segments demonstrate a lower density of label when compared with wild type, whereas rhodopsin localizes most densely to the apical part of the photoreceptor in IFT88 mutants. Scale bar: 2 µm in A-C; 200 nm in D,E; 0.75 µm in F; 1 µm in G,H.





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