spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 5


Fig. 5. IFT20 and IFT88 localize to the base of the cilium in IFT57 mutant rods. (A) Wild-type rods expressing an IFT20-GFP transgene exhibit IFT20 localization at the base of the cilium (acetylated tubulin), where it colocalizes with acetylated tubulin (yellow; arrowhead). As the embryos do not carry an integrated transgene, only a small number of rod photoreceptors in the retina exhibit expression. (B) Loss of IFT57 does not affect IFT20-GFP localization to connecting cilia (arrowhead). Arrow indicates the connecting cilia in a neighboring cell that does not express the IFT20-GFP transgene. (C,D) In the presence or absence of IFT57, IFT20-GFP localized to the Golgi apparatus, where it colocalizes with syntaxin 6 (arrowhead). (E) IFT88 localizes to the base of the connecting cilium (arrowhead) in wild-type photoreceptors as well as in (F) IFT57 mutant photoreceptors. Abbreviations: AT, acetylated tubulin; Syn-6, syntaxin 6. Scale bar: 10 µm in A,B,E,F; 40 µm in C,D.





Right arrow Return to article